a AFM image of monomerized CYP11A1 obtained using ultrasharp AFM probe; b cross-section, shown in (a); c density of distribution with height and volume of imaged CYP11A1. Experimental conditions were as follows: 100 μM CYP11A1 monomerized in 50 mM KP, pH 7.4, containing Emulgen 913 (12%). For AFM visualization, the samples were diluted to obtain 1 μM CYP11A1 in 50 mM KP with 0.5% Emulgen 913, pH 7.4, and immediately placed onto the mica surface. T = 25°C. Tapping mode. AFM cantilevers were NSG01_DLC (NT-MDT, Russia).
Ivanov et al. Nanoscale Res Lett 2011 6:54 doi:10.1007/s11671-010-9809-5