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Open Access Highly Accessed Nano Express

Biofunctionalization of zinc oxide nanowires for DNA sensory applications

Raphael Niepelt1*, Ulrich C Schröder1, Jana Sommerfeld1, Irma Slowik1, Bettina Rudolph2, Robert Möller2, Barbara Seise2, Andrea Csaki2, Wolfgang Fritzsche2 and Carsten Ronning1*

Author Affiliations

1 Institute of Solid State Physics, Friedrich-Schiller-Universität, Max-Wien-Platz 1, 07743 Jena, Germany

2 Institute of Photonic Technology (IPHT), PO Box 100239, 07702 Jena, Germany

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Nanoscale Research Letters 2011, 6:511  doi:10.1186/1556-276X-6-511

Published: 25 August 2011

Abstract

We report on the biofunctionalization of zinc oxide nanowires for the attachment of DNA target molecules on the nanowire surface. With the organosilane glycidyloxypropyltrimethoxysilane acting as a bifunctional linker, amino-modified capture molecule oligonucleotides have been immobilized on the nanowire surface. The dye-marked DNA molecules were detected via fluorescence microscopy, and our results reveal a successful attachment of DNA capture molecules onto the nanowire surface. The electrical field effect induced by the negatively charged attached DNA molecules should be able to control the electrical properties of the nanowires and gives way to a ZnO nanowire-based biosensing device.

Keywords:
nanowires; zinc oxide; functionalization; fluorescence microscopy; sensor technology