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Open Access Nano Express

A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA

Wang Yu-Hong1, Chen Rui2 and Li Ding3*

Author Affiliations

1 Emergency Department, General Hospital of Beijing Military Area of PLA, Beijing 100700, China

2 The Department of Blood Transfusion, Xijing Hospital, The Fourth Military Medical University, Xian 710032, China

3 Center of Biological Diagnosis and Therapy, No. 261 Hospital of PLA, Beijing 100094, China

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Nanoscale Research Letters 2011, 6:461  doi:10.1186/1556-276X-6-461

Published: 20 July 2011

Abstract

Background

The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present, the diagnostic test of human papillomavirus (HPV) relies exclusively on molecular test. Hereon, we report a rapid and facile quantum dots (QDs) and superparamagnetic nanoparticle-based hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution, and total time for detection is no more than 1 h.

Methods

The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes, and the results were compared with type-specific polymerase chain reaction (PCR) method.

Results

The statistic analysis suggests that there is no significant difference between these two methods. Furthermore, this method is much quicker and easier than the type-specific PCR method.

Conclusion

This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage, especially mass epidemiological screening.

Keywords:
HPV; DNA; quantum dots; superparamagnetic nanoparticles; hybridization; cervical cancer