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Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy

Feiyue Xing1*, Jiongkun Wang1, Mingqian Hu2, Yu Yu34, Guoliang Chen1 and Jing Liu5*

Author Affiliations

1 Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China

2 Department of Chemistry, Jinan University, Guangzhou 510632, China

3 Department of Immunology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA

4 Department of Blood and Marrow Transplantation, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA

5 Department of Stomatology, Jinan University, Guangzhou 510632, China

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Nanoscale Research Letters 2011, 6:455  doi:10.1186/1556-276X-6-455

Published: 16 July 2011

Abstract

A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs.

Keywords:
dendritic cell; nanostructure; adhesion force; comparison