Confocal laser scanning microscopy (CLSM) images of HeLa cells after 2 h incubation with coumarin-6-loaded5% DMAB-modified PLGA-TPGS nanoparticles at 37.0°C. The cells were stained by propidium iodide (red) and the coumarin-6-loaded nanoparticles are green. The cellular uptake is visualized by overlaying images obtained by white light, FITC filter and PI filter: upper-left image from FITC channel; upper-right image from transmitted light channel; lower-left image from PI channel; lower-right image from combined transmitted light channel, PI channel and FITC channel.
Chen et al. Nanoscale Res Lett 2011 6:4 doi:10.1007/s11671-010-9741-8