Plasma OVA-specific IgE Ab responses after intranasal exposure to OVA plus silica particles. (a) BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus silica particles (250 μg/mouse) on days 0, 1, and 2. (b) BALB/c mice were intranasally exposed to PBS (vehicle control), OVA alone or OVA plus the designated dose of nSP30 or nSP70 on days 0, 1, and 2. Plasma was collected on day 21 and analyzed by ELISA to assess (a) the relationship between silica particle size and OVA-specific IgE Ab responses and (b) the dose-response effect of nSP30 and nSP70 on OVA-specific IgE Ab levels. N.D., not detected. Data are presented as mean ± SEM (n = 8 to 13; *P < 0.05 vs OVA alone).
Yoshida et al. Nanoscale Research Letters 2011 6:195 doi:10.1186/1556-276X-6-195