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Open Access Nano Express

Protein Functionalized Nanodiamond Arrays

YL Liu and KW Sun*

Author affiliations

Department of Applied Chemistry, National Chiao Tung University, 30010, Hsinchu, Taiwan

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Citation and License

Nanoscale Research Letters 2010, 5:1045-1050  doi:10.1007/s11671-010-9600-7

Published: 14 April 2010

Abstract

Various nanoscale elements are currently being explored for bio-applications, such as in bio-images, bio-detection, and bio-sensors. Among them, nanodiamonds possess remarkable features such as low bio-cytotoxicity, good optical property in fluorescent and Raman spectra, and good photostability for bio-applications. In this work, we devise techniques to position functionalized nanodiamonds on self-assembled monolayer (SAMs) arrays adsorbed on silicon and ITO substrates surface using electron beam lithography techniques. The nanodiamond arrays were functionalized with lysozyme to target a certain biomolecule or protein specifically. The optical properties of the nanodiamond-protein complex arrays were characterized by a high throughput confocal microscope. The synthesized nanodiamond-lysozyme complex arrays were found to still retain their functionality in interacting with E. coli.

Keywords:
Nanodiamond; Biosensor; Self-assembled monolayer