Table 2

Comparison of several measurements of antibody-based protein–protein interactions by AFM
Molecular partners Substrate AFM tip Unbinding force (pN)
Human IgG/rat anti-human IgG [present study] Rat anti-human IgG Human IgG 144 ± 11
Human serum albumin (HSA)/anti-HSA [34] HSA Anti-HSA 244 ± 22
Sendai-purple membrane (Sendai-PM)/anti-Sendai antibody [2] Sendai-PM Anti-Sendai antibody 70–170
Angiogenin/anti-angiogenin antibody IgG [35] Anti-angiogenin antibody IgG Angiogenin 1029 ± 63
Spore coat protein CotA/anti-CotA [36] CotA Anti-CotA 55 ± 2
Collagen/collagen antibody [37] Collagen Collagen antibody 90 ± 40
HSA/anti-HSA [38] Anti-HSA HSA 88–94

Lv et al.

Lv et al. Nanoscale Research Letters 2010 5:1032-1038   doi:10.1007/s11671-010-9598-x

Open Data