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Open Access Nano Express

Biomolecular Nano-Flow-Sensor to Measure Near-Surface Flow

Sang-Wook Lee1, Haruyuki Kinoshita1, Hiroyuki Noji2, Teruo Fujii1 and Takatoki Yamamoto3*

Author Affiliations

1 Institute of Industrial Science, University of Tokyo, Fw-601 4-6-1 Komaba Meguro-ku, Tokyo, 153-8505, Japan

2 The Institute of Science and Industrial Research, Osaka University, 8-1 Mihogaoka-Ibaraki, Osaka, 567-0047, Japan

3 Department of Mechanical and Control Engineering, Tokyo Institute of Technology, Ishikawadai 1-314, Ookayama 2-12-1 Meguro-ku, Tokyo, 152-8550, Japan

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Nanoscale Research Letters 2009, 5:296-301  doi:10.1007/s11671-009-9479-3

Published: 14 November 2009

Abstract

We have proposed and experimentally demonstrated that the measurement of the near-surface flow at the interface between a liquid and solid using a 10 nm-sized biomolecular motor of F1-ATPase as a nano-flow-sensor. For this purpose, we developed a microfluidic test-bed chip to precisely control the liquid flow acting on the F1-ATPase. In order to visualize the rotation of F1-ATPase, several hundreds nanometer-sized particle was immobilized at the rotational axis of F1-ATPase to enhance the rotation to be detected by optical microscopy. The rotational motion of F1-ATPase, which was immobilized on an inner surface of the test-bed chip, was measured to obtain the correlation between the near-surface flow and the rotation speed of F1-ATPase. As a result, we obtained the relationship that the rotation speed of F1-ATPase was linearly decelerated with increasing flow velocity. The mechanism of the correlation between the rotation speed and the near-surface flow remains unclear, however the concept to use biomolecule as a nano-flow-sensor was proofed successfully.

(See supplementary material 1)

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Keywords:
Microfluidics; Near-surface; F1-ATPase; Micro-PIV; Flow-sensor