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Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors

Min Zhang, Ya-Nan Xue, Min Liu, Ren-Xi Zhuo and Shi-Wen Huang*

Author Affiliations

Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, 430072, People’s Republic of China

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Nanoscale Research Letters 2010, 5:1804-1811  doi:10.1007/s11671-010-9716-9

Published: 11 August 2010


An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense foreign plasmid DNA to form nanosized polycationic micelles/DNA polyelectrolyte complexes with positive charges, which transfected 293T cells with high efficiency. Under optimized conditions, the transfection efficiencies of polycationic micelles/DNA complexes are comparable to, or even higher than that of commercially available branched PEI (Mw 25 kDa).

Gene delivery; Polycationic micelles; Biocleavable