Open Access NANO EXPRESS

Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies

Juliana S Almeida1, Fernanda Lima2, Simoní Da Ros2, Luis OS Bulhões3, Leandro M de Carvalho12 and Ruy CR Beck4*

Author Affiliations

1 Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Av. Roraima, 1000, Santa Maria, RS, 97105-900, Brazil

2 Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

3 Departamento de Química, Universidade Federal de São Carlos, São Carlos, SP, 13565-905, Brazil

4 Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90610-000, Brazil

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Nanoscale Research Letters 2010, 5:1603-1610  doi:10.1007/s11671-010-9683-1

Published: 15 July 2010

Abstract

The improvement of the rutin photostability and its prolonged in vitro antioxidant activity were studied by means of its association with nanostructured aqueous dispersions. Rutin-loaded nanocapsules and rutin-loaded nanoemulsion showed mean particle size of 124.30 ± 2.06 and 124.17 ± 1.79, respectively, polydispersity index below 0.20, negative zeta potential, and encapsulation efficiency close to 100%. The in vitro antioxidant activity was evaluated by the formation of free radical ·OH after the exposure of hydrogen peroxide to a UV irradiation system. Rutin-loaded nanostructures showed lower rutin decay rates [(6.1 ± 0.6) 10−3 and (5.1 ± 0.4) 10−3 for nanocapsules and nanoemulsion, respectively] compared to the ethanolic solution [(35.0 ± 3.7) 10−3 min−1] and exposed solution [(40.1 ± 1.7) 10−3 min−1] as well as compared to exposed nanostructured dispersions [(19.5 ± 0.5) 10−3 and (26.6 ± 2.6) 10−3, for nanocapsules and nanoemulsion, respectively]. The presence of the polymeric layer in nanocapsules was fundamental to obtain a prolonged antioxidant activity, even if the mathematical modeling of the in vitro release profiles showed high adsorption of rutin to the particle/droplet surface for both formulations. Rutin-loaded nanostructures represent alternatives to the development of innovative nanomedicines.

Keywords:
Antioxidant activity; Nanocapsules; Nanoemulsions; Nanoparticles; Photostability; Rutin