Membrane Surface Nanostructures and Adhesion Property of T Lymphocytes Exploited by AFM
1 Chemistry Department, Jinan University, Guangzhou, 510632, Guangdong, People’s Republic of China
2 Institution for Tissue Transplantation and Immunology, Jinan University, Guangzhou, 510632, Guangdong, People’s Republic of China
3 Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510090, People’s Republic of China
4 The First Affiliated Hospital, Jinan University, Guangzhou, 510632, Guangdong, People’s Republic of China
Nanoscale Research Letters 2009, 4:942-947 doi:10.1007/s11671-009-9340-8Published: 5 June 2009
The activation of T lymphocytes plays a very important role in T-cell-mediated immune response. Though there are many related literatures, the changes of membrane surface nanostructures and adhesion property of T lymphocytes at different activation stages have not been reported yet. However, these investigations will help us further understand the biophysical and immunologic function of T lymphocytes in the context of activation. In the present study, the membrane architectures of peripheral blood T lymphocytes were obtained by AFM, and adhesion force of the cell membrane were measured by acquiring force–distance curves. The results indicated that the cell volume increased with the increases of activation time, whereas membrane surface adhesion force decreased, even though the local stiffness for resting and activated cells is similar. The results provided complementary and important data to further understand the variation of biophysical properties of T lymphocytes in the context of in vitro activation.