Open Access Nano Express

Subcellular Localization of Thiol-Capped CdTe Quantum Dots in Living Cells

Yu Zhang1, Lan Mi1, Rongling Xiong1, Pei-Nan Wang1*, Ji-Yao Chen2*, Wuli Yang3, Changchun Wang3 and Qian Peng4

Author Affiliations

1 Department of Optical Science and Engineering, Key Lab for Advanced Photonic Materials and Devices, Fudan University, Shanghai, 200433, China

2 Surface Physics Laboratory (National Key Lab) and Department of Physics, Fudan University, Shanghai, 200433, China

3 Department of Macromolecular Science and Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, 200433, China

4 Department of Pathology, The National Hospital-Norwegian Radium Hospital, University of Oslo, Montebello, Oslo, Norway

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Nanoscale Research Letters 2009, 4:606-612  doi:10.1007/s11671-009-9307-9

Published: 5 April 2009

Abstract

Internalization and dynamic subcellular distribution of thiol-capped CdTe quantum dots (QDs) in living cells were studied by means of laser scanning confocal microscopy. These unfunctionalized QDs were well internalized into human hepatocellular carcinoma and rat basophilic leukemia cells in vitro. Co-localizations of QDs with lysosomes and Golgi complexes were observed, indicating that in addition to the well-known endosome-lysosome endocytosis pathway, the Golgi complex is also a main destination of the endocytosed QDs. The movement of the endocytosed QDs toward the Golgi complex in the perinuclear region of the cell was demonstrated.

Keywords:
Cells; Confocal microscopy; Imaging; Quantum dots; Subcellular localization