Fatty Acid Binding Domain Mediated Conjugation of Ultrafine Magnetic Nanoparticles with Albumin Protein
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Nanoscale Research Letters 2008, 4:138-143 doi:10.1007/s11671-008-9213-6Published: 22 November 2008
A novel bioconjugate of stearic acid capped maghemite nanoparticle (γ-Fe2O3) with bovine serum albumin (BSA) was developed by taking recourse to the fatty acid binding property of the protein. From FT-IR study, it was found that conjugation took place covalently between the amine group of protein molecule and carboxyl group of stearic acid capped maghemite nanoparticle. TEM study further signified the morphology of the proposed nanobioconjuagte. The binding constant of nanoparticle with protein molecule was evaluated from the optical property studies. Also, magnetic measurement (M–H) showed retaining of magnetic property by significant values of saturation magnetization and other hysteretic parameters.