Figure 6.

Confocal laser scanning microscopy (CLSM) image of MCF-7 TAX30 cells after 2 h incubation with coumarin-6-loaded PCL/Pluronic F68 nanoparticles at 37.0 °C. The cells were stained by propidium iodide (red) and the coumarin-6-loaded nanoparticles aregreen. The cellular uptake is visualized by overlaying images obtained by white light, FITC filter, and PI filter: upper left image from FITC channel; upper right image from transmitted light channel; lower left image from PI channel; lower-right image from combined transmitted light channel, PI channel, and FITC channel