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Open Access Nano Express

Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage

ZR Guo12, CR Gu1, X Fan2, ZP Bian1, HF Wu1, D Yang1, N Gu2 and JN Zhang1*

Author Affiliations

1 The Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China

2 State Key Laboratory of Bioelectronics and Jiangsu Laboratory for Biomaterials and Devices, Southeast University, 210029, Nanjing, China

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Nanoscale Research Letters 2009, 4:1428-1433  doi:10.1007/s11671-009-9415-6

Published: 21 August 2009

Abstract

A facile, rapid, solution-phase method of detecting human cardiac troponin I for sensing myocardial damage has been described using gold nanorods-based biosensors. The sensing is demonstrated by the distinct change of the longitudinal surface plasmon resonance wavelength of the gold nanorods to specific antibody‚Äďantigen binding events. For a higher sensitivity, the aspect ratio of gold nanorods is increased up to ca 5.5 by simply adding small amount of HCl in seed-mediated growth solution. Experimental results show that the detecting limit of the present method is 10 ng/mL. Contrast tests reveal that these gold nanorods-based plasmonic biosensors hold much higher sensitivity than that of conventionally spherical gold nanoparticles.

Keywords:
Gold nanorod; Biosensor; Surface modification; Cardiac troponin I