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Open Access Nano Express

In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release

Jin Zhang1*, Lynne-Marie Postovit2, Dashan Wang3, Richard B Gardiner4, Richard Harris4, MuminMd Abdul1 and AnuAlice Thomas1

Author Affiliations

1 Department of Chemical and Biochemical Engineering, University of Western Ontario, London, ON, N6A 5B9, Canada

2 Department of Anatomy and Cell Biology, The Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada

3 National Research Council Canada, Institute for Chemical Process and Environmental Technology, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada

4 Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

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Nanoscale Research Letters 2009, 4:1297-1302  doi:10.1007/s11671-009-9395-6

Published: 25 July 2009

Abstract

Basic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration. However, bFGF is easily degraded in biologic systems. Mesoporous silica nanoparticles (MSNs) with well-tailored porous structure have been used for hosting guest molecules for drug delivery. Here, we report an in situ route to load bFGF in MSNs for a prolonged release. The average diameter (d) of bFGF-loaded MSNs is 57 ± 8 nm produced by a water-in-oil microemulsion method. The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay. The loading efficiency of bFGF in MSNs is estimated at 72.5 ± 3%. In addition, the cytotoxicity test indicates that the MSNs are not toxic, even at a concentration of 50 μg/mL. It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs, e.g. growth factors, to help blood vessel regeneration and potentiate greater angiogenesis.

Keywords:
In situ loading method; Protein release; Nanoparticles