Table 3

Effects of nano-TiO2on the amplification of cytokine mRNA of mouse by real-time PCR analysis after intraperitoneal injection with nano-anatase TiO2suspensions for consecutive 14 days
Cytokine Nano-anatase (mg/kg BW)
0 50 100 150 150-bulk
Refer-actin Ct 21.035 19.93 21.4249 21.8257 21.038
Copies 2.68E+07 5.64E+07 2.06E+07 1.57E+07 2.68E+07
nf-κb Ct 23.144 21.7009 22.4526 22.2415 23.0754
Relative copies 6.47E+06 1.71E+07 1.02E+07 1.19E+07 6.78E+06
Ratio ofnf-κb/actin 0.242 ± 0.012 0.303 ± 0.015* 0.497 ± 0.025** 0.756 ± 0.038** 0.253 ± 0.013
mif Ct 22.4026 21.7153 21.5595 21.5625 20.5766
Relative copies 1.07E+07 1.70E+07 1.88E+07 1.88E+07 3.65E+07
Ratio ofmif/actin 0.398 ± 0.020 0.634 ± 0.032** 0.913 ± 0.046** 1.194 ± 0.060** 0.647 ± 0.032**
il-1β Ct 24.2684 22.911 20.9252 22.2075 24.0418
Relative copies 3.04E+06 7.57E+06 2.89E+07 1.22E+07 3.54E+06
Ratio ofil-1β/actin 0.113 ± 0.006 0.481 ± 0.024** 0.511 ± 0.026** 0.590 ± 0.030** 0.132 ± 0.007*
il-6 Ct 25.076 22.6692 23.3343 22.8735 23.4873
Relative copies 1.76E+06 8.9lE+06 5.70E+06 7.77E+06 5.14E+06
Ratio ofil-6/actin 0.066 ± 0.003 0.158 ± 0.008** 0.276 ± 0.014** 0.494 ± 0.025** 0.192 ± 0.010**
crp Ct 23.9738 21.7188 23.152 22.907 23.0768
Relative copies 3.70E+05 1.69E+07 6.44E+06 7.59E+06 6.77E+06
Ratio ofcrp/actin 0.138 ± 0.007 0.300 ± 0.015** 0.312 ± 0.016** 0.482 ± 0.024** 0.253 ± 0.013**
tnf Ct 28.674 28.8403 28.659 28.3492 28.7297
Relative copies 1.56E+05 1.39E+05 1.58E+05 1.94E+05 1.50E+05
Ratio oftnf/actin 0.028 ± 0.001 0.052 ± 0.003** 0.076 ± 0.004** 0.123 ± 0.006** 0.056 ± 0.003
il-4 Ct 23.7878 22.2796 21.1993 21.3352 22.906
Relative copies 4.20E+06 1.16E+07 2.40E+07 2.19E+07 7.60E+06
Ratio ofil-4/actin 0.157 ± 0.008 0.205 ± 0.010** 0.895 ± 0.045** 1.392 ± 0.070** 0.369 ± 0.018**
π-10 Ct 28.5199 27.022 28.6367 28.1555 28.169
Relative copies 173E+05 4.75E+05 1.60E+05 2.21E+05 2.19E+05
Ratio ofπ-10/actin 0.065 ± 0.003 0.084 ± 0.004* 0.102 ± 0.005** 0.107 ± 0.005** 0.082 ± 0.004*

Ranks marked with an asterisk or double asterisks means it is significantly different from the control (no nano-anatase or bulk TiO2) at the 5 or 1% confidence level, respectively. Values represent mean ± SE,n = 5

Ma et al.

Ma et al. Nanoscale Research Letters 2009 4:1275-1285   doi:10.1007/s11671-009-9393-8

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