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The Acute Liver Injury in Mice Caused by Nano-Anatase TiO2

Linglan Ma, Jinfang Zhao, Jue Wang, Jie Liu, Yanmei Duan, Huiting Liu, Na Li, Jingying Yan, Jie Ruan, Han Wang and Fashui Hong*

Author Affiliations

Medical College of Soochow University, 215123, Suzhou, People’s Republic of China

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Nanoscale Research Letters 2009, 4:1275-1285  doi:10.1007/s11671-009-9393-8

Published: 1 August 2009


Although it is known that nano-TiO2or other nanoparticles can induce liver toxicities, the mechanisms and the molecular pathogenesis are still unclear. In this study, nano-anatase TiO2(5 nm) was injected into the abdominal cavity of ICR mice for consecutive 14 days, and the inflammatory responses of liver of mice was investigated. The results showed the obvious titanium accumulation in liver DNA, histopathological changes and hepatocytes apoptosis of mice liver, and the liver function damaged by higher doses nano-anatase TiO2. The real-time quantitative RT-PCR and ELISA analyses showed that nano-anatase TiO2can significantly alter the mRNA and protein expressions of several inflammatory cytokines, including nucleic factor-κB, macrophage migration inhibitory factor, tumor necrosis factor-α, interleukin-6, interleukin-1β, cross-reaction protein, interleukin-4, and interleukin-10. Our results also implied that the inflammatory responses and liver injury may be involved in nano-anatase TiO2-induced liver toxicity.

Mice; Nano-anatase TiO2; Liver; Inflammatory cytokines; Histopathological changes