Micro/Nanoscale Parallel Patterning of Functional Biomolecules, Organic Fluorophores and Colloidal Nanocrystals
1 Italian Institute of Technology (IIT), National Nanotechnology Laboratory, Via Barsanti 1, Arnesano, Lecce, 73010, Italy
2 National Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, University of Salento, Via Arnesano 16, Lecce, 73100, Italy
Nanoscale Research Letters 2009, 4:1222-1229 doi:10.1007/s11671-009-9386-7Published: 14 July 2009
We describe the design and optimization of a reliable strategy that combines self-assembly and lithographic techniques, leading to very precise micro-/nanopositioning of biomolecules for the realization of micro- and nanoarrays of functional DNA and antibodies. Moreover, based on the covalent immobilization of stable and versatile SAMs of programmable chemical reactivity, this approach constitutes a general platform for the parallel site-specific deposition of a wide range of molecules such as organic fluorophores and water-soluble colloidal nanocrystals.