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Open Access Nano Express

Fabrication of DNA micropatterns on the polycarbonate surface of compact discs

Zhen Wang* and Rong-Xian Li

Author Affiliations

Center for Advanced Materials & Biotechnology, Research Institute of Tsinghua University in Shenzhen, Shenzhen, 518057, P.R. China

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Nanoscale Research Letters 2006, 2:69-74  doi:10.1007/s11671-006-9032-6

The electronic version of this article is the complete one and can be found online at:


Received:24 October 2006
Accepted:8 November 2006
Published:8 December 2006

© 2006 to the authors

Abstract

DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as “anchor” to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.

Keywords:
DNA; Compact disc; UV/ozone; Hybridization

Nano Express

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