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Open Access Nano Express

In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics

Biswarup Saha1, Jaydeep Bhattacharya2, Ananda Mukherjee1, AnupKumar Ghosh3, ChittaRanjan Santra4, Anjan K Dasgupta5 and Parimal Karmakar1*

Author Affiliations

1 Department of Life Science and Biotechnology, Jadavpur University, Kolkata, 700 032, WB, India

2 Department of Microbiology, Vijoygarh Jyotish Roy College, University of Calcutta, Jadavpur, Kolkata, 700 032, WB, India

3 Department of Instrumentation Science, Jadavpur University, Kolkata, 700 032, WB, India

4 Department of Chemistry, Netaji Nagar Day College, NSC Bose Road, Regent Estate, Kolkata, 700 092, WB, India

5 Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700 019, WB, India

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Nanoscale Research Letters 2007, 2:614-622  doi:10.1007/s11671-007-9104-2

Published: 17 November 2007

Abstract

Bactericidal efficacy of gold nanoparticles conjugated with ampicillin, streptomycin and kanamycin were evaluated. Gold nanoparticles (Gnps) were conjugated with the antibiotics during the synthesis of nanoparticles utilizing the combined reducing property of antibiotics and sodium borohydride. The conjugation of nanoparticles was confirmed by dynamic light scattering (DLS) and electron microscopic (EM) studies. Such Gnps conjugated antibiotics showed greater bactericidal activity in standard agar well diffusion assay. The minimal inhibitory concentration (MIC) values of all the three antibiotics along with their Gnps conjugated forms were determined in three bacterial strains,Escherichia coli DH5α,Micrococcus luteusandStaphylococcus aureus. Among them, streptomycin and kanamycin showed significant reduction in MIC values in their Gnps conjugated form whereas; Gnps conjugated ampicillin showed slight decrement in the MIC value compared to its free form. On the other hand, all of them showed more heat stability in their Gnps conjugated forms. Thus, our findings indicated that Gnps conjugated antibiotics are more efficient and might have significant therapeutic implications.

Keywords:
Gold nanoparticles; Antibiotics; Dynamic light scattering; Transmission electron microscope; Scanning electron microscope; Minimal inhibitory concentration; Agar well diffusion